GENE CLUSTER INVOLVES IN BIOSYNTHESIS OF SAFRACIN AND APPLICATION THEREOF IN GENETIC ENGINEERING

摘要

FIELD: medicine.;SUBSTANCE: there is recovered a fragment of genomic DNA Pseudomonas fluorescent A2-2, including full-size gene cluster of biosynthesis of safracin (A and B) analysis of which showed the presence of several "OPC" arranged in two operons: sacABCDEFGH and sacIJ. Expression of nucleic acid containing full gene cluster of safracin in a heterosystem enabled producing recombinant forms of natural safracins A and B.;EFFECT: removal or malfunction of separate genes found in operons, and applications of the produced modified forms of nucleic acid in the recombinant DNA technology have resulted in synthesised analogues of safracin to be used as an antimicrobial or antitumour agent, and also in synthesis of ecteinascedine compounds.;20 cl, 9 dwg, 7 ex