RECOMBINANT PLASMID, PROVIDING FOR EXPRESSION OF GENE OF EXTRACELL RIBONUCLEASE Zinnia elegans ZRNaseII IN TRANSGENIC PLANTS (VERSIONS) AND METHOD FOR PRODUCTION OF VIRUS-RESISTANT FORMS OF PLANTS

摘要

FIELD: agriculture.;SUBSTANCE: plasmid pC27RNS contains DNA of coding part of gene of extracell ribonuclease Zinnia elegans ZRNasell under control of inducible MAS2' -promotor. Plasmid pBiRNS contains DNA of coding part of gene of extracell ribonuclease Zinnia elegans ZRNasell under control of inducible 35S-promotor. Plasmid pC27RNS or pBiRNS is transferred into strain Agrobacterium tumefaciens. Produced agrobacteria are used to transform plant material by cocultivation with Agrobacteria and further selection of transgentic plants at selective medium.;EFFECT: expression of gene of extracell ribonuclease Zinnia elegans ZRNasell in plants gives them imprived resistance to virus infections.;3 cl, 3 dwg, 3 tbl, 5 ex