METHOD FOR ESTIMATING ADHESION STAPHYLOCOCCUS SPP TO HAEMOPROTEINS

摘要

FIELD: medicine.;SUBSTANCE: invention is intended for a quantitative estimation of adhesive properties of staphylococci recovered from a human and environment objects, with its bacteriological identification and for characterisation of its virulent properties in vitro by the adhesion to biopolymeric molecules. The staphylococcus culture being investigated is incubated in wells of a polystyrene plate with immobilised haemoproteins (muscle haemoglobin, haemoglobin) in a nutrient medium 199 for 1 hour; thereafter, nonadherent bacteria are removed by triple washing in the medium 199 with Twin-20, and a number of adhesive staphylococci is estimated by harvest cells and shown by optical density of the medium and by counting the cells sown on the dense nutrient medium from a series of tenfold cultivations, or by analysing the adhesive strains for the activity of bacterial enzymes.;EFFECT: good results reproducibility, high sensitivity of the method, ease of the reaction directly in wells of the polystyrene plate and possible express estimation of adhesive capacity of strains.;3 tbl, 3 ex