FIELD: medicine.;SUBSTANCE: there is offered a method of discrimination and calculation of at least two populations of biological elements - carriers of specific signs, probably presented in a sample. The method provides the use of three different probes, each of which is specifically fixed with one of the populations of biological elements which are required to be detected. Each probe itself becomes detectable due to its proper marker, and two different markers specified have two emission spectra containing at least one common part (overlapping emission spectra), and the third one has the emission spectrum which essentially contain no common parts with two others (nonoverlapping spectrum).;EFFECT: invention allows definitely detecting three populations of biological elements by using only two detection devices that assumes that two populations of biological elements will be detected by the same detection device.;9 cl, 2 ex, 1 tbl, 4 dwg
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