A method and device for biochemical detection of toluene in the air

摘要

A method for biochemical detection of toluene in the air, with the method on the basis of the biosensor technology a selective quantitative determination takes place, characterised in that on a for uv - light transparent carrier (a) a lyogel such sol - gel - layer (b) with enzymes (c) of the toluene - dioxygenase (death) - complex consisting of three individual enzymes, of the nadh - ferredoxin - reductase_tol, the ferredoxin_tol and an iron - sulphur - protein (isp_tol), and the coenzyme nadh (d) or its oxidized form of nad⁺ is applied, to prepare the lyogel that the left-hand side of the layer a defined volume ratio between the enzyme solution and precursors in the extension is set, the layer (b) of an air sample, respectively with analyte contained therein is subjected, further the layer (b) - by uv light in the wavelength range of from 310 – 370 nm, is irradiated in the analyte molecules penetrate the layer (b), where it is biochemically by the immobilized enzymes under changing the nadh - concentration, are reacted with changing the nadh - concentration, the absorption of the layer in the region between 310 – 370 nm changed over time, the change in the absorption by means of optoelectronic components..