method for detection and mitigation of the risk for breast cancer by the micronucleus test in zweikernigen cells

摘要

Determining the risk of developing breast cancer based on spontaneous development of micronuclei in mitogen-treated lymphocyte cultures, is new. Method for determining the risk of developing breast cancer comprising: (a) establishing a lymphocyte culture from heparin-treated peripheral blood; (b) mitogenic stimulation within 6 hours of establishing the culture; (c) adding a cytokinesis inhibitor 40-50 hours after establishment; (d) interrupting culture 12-24 hours after (c) by centrifuging, hypotonic treatment then preparation of a fixed cell suspension; (e) making a cytological preparation; (f) determining the micronucleus rate (MNR) in binuclear cells; and (g) determining the risk by comparing spontaneous MNR in the test sample with that in controls.