expression of class ii and class iii cells mannosidase mannosidase in lower eukaryotes

摘要

A method for producing human-like glycoproteins by expressing a Class 2 ±-mannosidase having a substrate specificity for Man±1,3 and Man±1,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.