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Methods and vectors for transformation of plastids and marker excision using site-specific recombination
Methods and vectors for transformation of plastids and marker excision using site-specific recombination
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机译:使用位点特异性重组技术转化质体和标记切除的方法和载体
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摘要
The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.
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