Photoacoustic analysis method and a photoacoustic spectrometer for measuring the concentration of analyte in the subject organization

摘要

PROBLEM TO BE SOLVED: To compensate an influence of a bulk tissue characteristic to noninvasive measurement of an analysis object concentration in an object tissue, relative to an ultrasonic pulse generated in the object tissue in a specimen and propagated.;SOLUTION: This analysis method has a step a for irradiating a specimen portion with a near infrared laser pulse from laser sources 15-18, and generating the ultrasonic pulse in the object tissue; a step b for detecting the ultrasonic pulse by a detector 32, and generating the first signal; a step c for generating the ultrasonic pulse in the object tissue by using a laser pulse having the same wavelength and energy as in the step b during an arrangement period of a light absorbing material between the laser sources and the specimen portion so that the ultrasonic pulse is generated in the light absorbing material, propagated through the tissue, and detected by the detector; a step d for detecting the ultrasonic pulse generated in the light absorbing material, and generating the second signal; a step e for calculating the intensity ratio between the first and second signals relative to each wavelength; a step f for calculating an absorption coefficient of the analysis object from the intensity ratio and an optical characteristic of the light absorbing material; and a step g for calculating the concentration of the analysis object from the calculated absorption coefficient, and calculating a molar absorption coefficient of the analysis object.;COPYRIGHT: (C)2008,JPO&INPIT