Recovery of the plasmid in aqueous two-phase system

摘要

A process for the purification of plasmid DNA, comprising the steps of: providing a composition comprising a second polymer immiscible with the first polymer and a first polymer having an inverse solubility, aqueous solution containing a plasmid DNA solution raised to a temperature lower the steps of contacting with the steps of isolating the aqueous phase is subjected to phase separation, than the temperature at which the plasmid DNA is degraded greater than the cloud point of the first polymer, the temperature of the phase isolated and a step of isolating the aqueous phase is, thus formed subsequently. In addition, it includes a kit for the purification of plasmid DNA as described above.