And their use fluorescent proteins rapidly maturing

摘要

Nucleic acid composition to code (and variants thereof), as well as to provide the protein encoded by the same thing that non-aggregated and variants fluorescent protein rapidly maturing. The protein of interest with a fluorescent, this feature arises from the interaction of two or more residues of the protein. In some embodiments, (Cnidarian), or Hana-chu such non-sea pen (Pennatulacean) protein of the present invention, from either (Shipen (sea pen)) of the species non-bioluminescent cnidarians anthozoan such as (Anthozoan) I further has a feature that is a variant of the wild-type protein was obtained. For some embodiments, the protein of the present invention is a mutant wild-type beetles sea anemone (Discosoma) species of "red" fluorescent protein. Is of interest also variants thereof or proteins, similar protein and the specific substantially. In addition, peptides encoding it and fragments of nucleic acids and proteins of the present invention, transformed cells, and antibodies to the transformed tissue is also provided. Nucleic acid composition and protein composition of the present invention is used in a variety of application was said. Finally, (such as a kit comprising a nucleic acid composition of the present invention, for example) kit for use in such applications is provided.