Nanoscale electronic detection system and method of manufacturing the same

摘要

A new, extremely sensitive, and rapid electronic detection method for direct detection of hybridized genomic targets to specific probes on the microarray is proposed. The method consists of fast electronic accumulation of the DNA target on a particular electrode site at the micro-electrode array, sequential electronic hybridization of oligonucleotide labeled metallic (nano)particles on the target DNA and monitoring the electrochemical AC impedance changes at the electrode site. The method is enhanced by electroplating over the DNA target which serves as the metallization template and over the particles which provide seeds for rapid electroplating. The AC impedance changes are monitored during the electroplating over the DNA target and between the array electrodes sites. The signal in the absence and presence of the target DNA is a difference between "no connection" and a "short" between the array electrodes