Simultaneous sequence analysis of the amino and carboxyl termini

摘要

By deriving the amino acid sequence at the amino terminus and carboxyl by mass spectrometry, the present invention relates to a novel method for identifying a polypeptide. Step of dissociation (eg, z 4) the peptide precursor ions by electron transfer dissociation-induced anion, highly charged, then, to remove these reagents, the present method, proton transfer-induced cation second type of and a step of introducing. A series of possible to reduce z- type fragments and c highly charged by converting +1 charge state mainly ETD products, and easily interpreted to reveal each sequence of amino and carboxyl termini to generate the m / z spectrum comprising z- type fragment ions c, and adjusting the PTR reaction duration second. [Selection Figure Figure 5