Conditional knockout cell of centromere localization protein gene

摘要

PPROBLEM TO BE SOLVED: To provide a centromere-localized protein gene knockout cell. PSOLUTION: This tetracycline-responding CENP-K or CENP-M knockout cell, having a gene of CENP-K or CENP-M inserted into the downstream of a promoter responding to tetracycline, lacking functions on the chromosome of the protein CENP-K or CENP-M which comprises a specific amino acid sequence or the specific amino acid sequence whose one or several amino acids are deleted, replaced or added, is localized in a centromere, and has a binding property to CENP-H or CENP-I. PCOPYRIGHT: (C)2007,JPO&INPIT