Cultured myocytes capable of measuring membrane translocation activity of insulin-responsive sugar transporter

摘要

PPROBLEM TO BE SOLVED: To provide a cultured muscle cell extremely suitable for measurement of membrane transfer activity of GLUT4 dependent to external stimulation of insulin or the like; and to provide a method for measuring the membrane transfer activity of the GLUT4 using the cell. PSOLUTION: The differentiation-type cultured muscle tube cell constructively expressing the recombinant GLUT4 is obtained by carrying out the mixed culture of a wild myoblast and a recombinant myoblast constructively expressing the recombinant GLUT4 having a label material at the extracellular site. The method for measuring the membrane transfer activity of the recombinant GLUT4 uses the cell. Especially, the method for measuring the activity for taking sugar in, dependent on the external stimulation is provided. PCOPYRIGHT: (C)2007,JPO&INPIT