Separation method for immature fish germ cells using surface immature germ cell specific protein

摘要

The present invention provides a gene, polynucleotide, polypeptide, an antibody that specifically binds to the polypeptide, which is specifically expressed in immature germ cells of fish, and an GFP gene by using the antibody. It is an object of the present invention to provide a method for isolating immature germ cells from donor fish without introducing such a marker gene, and a method for identifying immature germ cells derived from fish. The present invention is characterized by using the polypeptide shown in the following (A), (B) or the like, or a polynucleotide encoding the same. (A) a polypeptide comprising the amino acid sequence represented by amino acid numbers 1-1705 in SEQ ID NO: 2; (B) substitution of one or several amino acids in the amino acid sequence represented by amino acid numbers 1-1705 in SEQ ID NO: 2, A polypeptide consisting of an amino acid sequence deleted, inserted, or added and specifically expressed in immature germ cells derived from fish: