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Isolated manner of the poly medicine tolerance green pus germs and concentration of the creation mannered null NAC agar nutrient medium of the poly

机译:多药抗性绿色脓菌的分离方式和多菌种无效NAC琼脂营养培养基的创造浓度

摘要

PROBLEM TO BE SOLVED: To provide a method for quickly isolating multiple-drug-resistant Pseudomonas aeruginosa (MDRP) without causing an omission in picking-up, and to provide a selective culture medium therefor.;SOLUTION: Provided are the method for isolating MDRP, comprising dissolving 35.8 g of a NAC agar culture medium (dried powder), 16 mg of ciprofloxacin hydrochloride, and 32 mg of amikacin in 1,000 mL of water, boiling the solution, cooling the boiled solution to 60°C, adding 0.032 g of an imipenem cilastatin sodium equivalent weight mixture to make an agar plate culture medium, smearing the culture medium with a sample such as a patient specimen, culturing it at a constant temperature of 36±1°C for 24 hours, and checking the presence or absence of a yellow to yellowish green colony on the culture medium; and the selective culture medium.;COPYRIGHT: (C)2009,JPO&INPIT
机译:解决的问题:提供一种快速分离多药耐药性铜绿假单胞菌(MDRP)的方法,并且不会造成捡拾遗漏,并为其提供选择性培养基。解决方案:提供了一种分离MDRP的方法包括将35.8 g NAC琼脂培养基(干燥粉末),16 mg环丙沙星盐酸盐和32 mg阿米卡星溶解在1,000 mL水中,将溶液煮沸,将煮沸的溶液冷却至60°C,加入0.032 g用亚胺培南西司他丁钠当量混合物制成琼脂平板培养基,在培养基上涂上样品,例如患者标本,在36±1℃的恒温下培养24小时,然后检查是否存在在培养基上出现黄色至淡黄色绿色菌落;和选择性培养基。;版权所有:(C)2009,日本特许厅&INPIT

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