(57) Abstract This invention the measles virus (MV) and the RS virus (RSV) the attenuation the way and/or includes the feature regarding the production method of the virus like the infection characteristic virus, the mono negative virus (Mononegavirales) with it regards the revised method for producing the one chain RNA virus of negative sense of the non segmentation of the eye which is named. Including the nuclear acid molecule where the mono negative virus eye (Order Mononegavirales) from one production method of the rearrangement virus becomes and, is isolated, (a) in the minimum of 1 host cells, the (i) mono negative virus eye (Order Mononegavirales) including the polynucleotide arrangement which the genome or the anti genome of the one chain RNA virus of negative sense of the non segmentation the cord/code is done, including the nuclear acid molecule where the minimum of 1 kinds which the cord/code it does the protein which operates the trance which is necessary for the copying vector, and (ii) suffering package conversion, copying and the duplication which become are isolated, including the manifestation vector of the minimum of 1 kinds which become, the transfection of the rescue composition which becomes; Although also these vector production of the revelation and rearrangement virus is made possible, under sufficient condition execute in the host cell; Although (b) the rescue composition which the transfection is done is rearranged, and collection of the virus is made to increase, heat to effective thermal shock temperature under sufficient condition; And, depending upon when (c) including the fact that the rearrangement virus which it occurs is harvested, it becomes.
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