首页>
外国专利>
Filamentous single-chain trispecific antibody by gene recombination of anti-CEA, anti-CD3 and anti-CD28
Filamentous single-chain trispecific antibody by gene recombination of anti-CEA, anti-CD3 and anti-CD28
展开▼
机译:抗CEA,抗CD3和抗CD28基因重组的丝状单链三特异性抗体
展开▼
页面导航
摘要
著录项
相似文献
摘要
This invention refers to a linear single chain recombinant trispecific antibody (scTsAb). This trispecific antibody is composed of an anti-carcinoembryonic antigen (CEA), an anti-CD3, and an anti-CD28 (CEA-scTsAb). More specifically, a Fc linking peptide fuses the anti-CEA single chain fragment (scFv) to the anti-CD3 scFv, and an HSA linking peptide fuses the anti CD3 scFv to the anti-CD28 heavy variable chain (V H ) in tandem. A c-myc-tag and/or (His) 6-tag is attached to the C terminal of the CEA-scTsAb either alternatively or simultaneously. This invention also refers DNA sequence coding for the said scTsAbs, plasmids containing the said sequences, and host cells containing the said plasmids. These invented scTsAbs are intended for use in the development of therapeutic drug candidate for treatment of human colon cancer.
展开▼