HIGHLY POROUS SOLID MATERIAL MADE OF BIODEGRADABLE POLYMER AND METHOD OF FABRICATING, PROCESSING, AND CELL-SEEDING THE SAME

摘要

[Problems] A purpose of the invention is to provide highly porous 3D scaffolds made of biodegradable polymer such as poly-lactic acid, which can be preferably used for cell culture.;Another purpose of this invention is to provide a method for seeding cells homogeneously inside the above mentioned 3D highly porous scaffolds.;Another purpose of this invention is to provide a top-down processing method for obtaining planar porous 2D scaffolds starting the above mentioned highly porous scaffolds to be used as building blocks for thick tissue reconstruction.;[Solution] Highly porous scaffolds made of biodegradable polymer such as poly-lactic acid can be fabricated by following steps:(i) biodegradable polymer is dissolved in dioxane at the defined concentration;(ii) a vessel containing the solution is mounted on a metal plate thermally driven by a cold finger to a defined temperature below 10° C., and then the vessel is kept on the plate, until the solution undergoes complete phase separation; and(iii) the obtained solids are immersed into an alcoholic aqueous solution to leach out the dioxane.