CAPILLARY TWO- OR MULTI-DIMENSIONAL ELECTROPHORESIS IN A SINGLE CAPILLARY

摘要

The present invention relates to a method for separating the components of a mixture M by electrophoresis in a single capillary, wherein: (A) the compounds of the mixture M are introduced and migrate inside the capillary according to the capillary electrophoresis technique; (B) a fraction F of the compounds separated in step (A) is isolated in the capillary and said isolated fraction F is forced to migrate towards the vicinity of one end of the capillary while maintaining this fraction inside the capillary; (C) upon the application of a pressure difference between the ends of the capillary, a second separation medium MS² different from the first separation medium MS¹ of step (A) is introduced into the capillary through the end of the capillary in the vicinity of which the fraction F is located, whereby the fraction F migrates to the other end of the capillary while filling the capillary with the separation medium MS²; and (D) an capillary electrophoresis is carried out in the capillary thus filled with the separation medium MS², using the medium MS² as the separation medium.