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Method for recloning Chinese hamster ovary (CHO) cells

机译:克隆中国仓鼠卵巢(CHO)细胞的方法

摘要

A new method for selecting clones and recloning mammalian cells which are of importance for the production of biopharmaceuticals, preferably hamster or mouse myeloma cells, with a high degree of automation and throughput. The invention relates to methods of depositing and replicating single cell clones of the cells in question. The invention also relates to methods of preparing proteins using cells which have been obtained and replicated by single cell deposition as well as compositions which allow the replication of single cells.
机译:一种新的选择克隆和克隆哺乳动物细胞的新方法,这些方法对于生产生物药物具有重要意义,尤其是仓鼠或小鼠骨髓瘤细胞,具有高度的自动化和高通量。本发明涉及沉积和复制所述细胞的单细胞克隆的方法。本发明还涉及使用已经通过单细胞沉积获得并复制的细胞制备蛋白质的方法,以及允许单细胞复制的组合物。

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