Method for humanizing Antibodies against Interleukin 10 (il-10)

摘要

Claim 1: a method to identify a sequence of germline aceptora to a Humanized Antibody.Characterized in that it comprises the steps of: a) densify a Human Antibody does not have the desired Biological activity; (b) to determine the amino acid sequence of the VH and VL Domains not Human Antibody and (c) the sequence of Human Antibody does not compare with a group of sequences of L Human Germ line.Where the comparison includes the substeps: (1) assign Sequence Numbers of residues from sequences of VH and VL Domains not Human; 2) to delineate the Regions CDR and FR in the sequence; 3) assign a numerical result default in each position for which the residual sequence S of Human and Human Germ line or are identical and 4) All the results for total WasteGenerate a score for each sequence of Human Germ line; and (d) to identify the sequence of Human Germ line with the result of total waste as the highest aceptora Germ Line sequence.