method, parent cell line, differentiated cell and method for generating a differentiated cell from an embryonic stem cell (s)

摘要

METHOD, PROGENITIVE CELL LINE, DIFFERENTIATED CELL AND METHOD FOR GENERATING A DIFFERENTIATED CELL FROM AN EMBRYONIC STEM CELL which comprises: (a) providing an embryonic stem cell (ES); and (b) establishing a progenitor cell line from the embryonic stem cell; the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived from the established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell lineage.