Method for concentrating, purifying and removing proteu00ecna priu00c8nica

摘要

b method to concentrate, purify and remove protein priu00c8nica. d , the present invention relates to a method for concentrating and / or purify prion protein PrP ^ SC ^ with the prion protein PrP ^ SC ^ in contact with Sepharose under conditions which allow the connection specific and high affinity of Sepharose prion protein PrPSc. Removing the proteins not priu00f3nio non connected with the Sepharose,And the same method to remove prion protein PrP ^ SC ^ of bodily fluids and the fluids in contact with Sepharose under conditions which allow specific and high affinity binding of prion protein PrP ^ SC Sepharose ^ and removing the body fluid of This Sepharose.In addition, the present invention relates to a method to separate and / or prion protein PrP ^ SC ^ enrich from protein PrP ^ C ^By placing the prion protein PrP ^ SC protein PrP ^ C ^ ^ and in contact with a Sepharose modified with ligand under conditions that allow for the specific and high affinity binding of part of Sepharose prion protein PrP ^ SC ^ and the connection part of the ligand for the Sepharose to P. Roteu00ednas PrP ^ C ^By adding a release agent selective protein linked to the Sepharose under conditions that allow the release of protein and non prion protein PrP ^ C ^ part of the ligand for the Sepharose, but not the release of prion protein PrPSc, and removing the proteins. The priu00f5nicas and PrP ^ C ^ of the Sepharose protein.Another aspect of the present invention refers to the use of the above methods to concentrate, purify and / or remove prion protein PrP ^ SC ^.