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Isolated polypeptides based on the neutralizing epitope of the p17 protein from HIV suitable for vaccines as well as neutralizing anti-p17 antibodies that specifically recognize said neutralizing epitope
Isolated polypeptides based on the neutralizing epitope of the p17 protein from HIV suitable for vaccines as well as neutralizing anti-p17 antibodies that specifically recognize said neutralizing epitope
New isolated polypeptides of the protein p17 of HIV are described, represented by the formula: NH2-X1-Gly-X2-X3-Leu-Asp-X4-Trp-Glu-X5-Ile-X6-Leu-Arg-COOH (SEQ ID NO:1) in which X1 is an amino acid residue selected from Ser and Arg; X2 is an amino acid residue selected from Gly, Glu and Ser; X3 is an amino acid residue selected from Glu, Lys, Asp and Arg; X4 is an amino acid residue selected from Arg, Ala, Lys, Thr, Ser, Glu, Asp and Gln; X5 is an amino acid residue selected from Lys, Arg and Ser; and X6 is an amino acid residue selected from Arg and Gln. These polypeptides, when administered to a subject, are able to evoke anti-p17 neutralizing antibodies and are therefore useful as vaccine or inoculum. Monoclonal and polycolonal anti-p17 antibodies which are able to neutralize the biological activity of this viral protein and to recognize its neutralization epitope in a specific manner are also described.
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机译:描述了HIV蛋白p17的新分离多肽,由下式表示:NH2-X1-Gly-X2-X3-Leu-Asp-X4-Trp-Glu-Xle-Xle-X6-Leu-Arg-COOH(SEQ ID NO:1),其中X1是选自Ser和Arg的氨基酸残基; X2是选自Gly,Glu和Ser的氨基酸残基; X3是选自Glu,Lys,Asp和Arg的氨基酸残基; X4是选自Arg,Ala,Lys,Thr,Ser,Glu,Asp和Gln的氨基酸残基; X5是选自Lys,Arg和Ser的氨基酸残基; X6是选自Arg和Gln的氨基酸残基。当将这些多肽施用于受试者时,它们能够引起抗p17中和抗体,因此可用作疫苗或接种物。还描述了能够中和该病毒蛋白的生物学活性并以特定方式识别其中和表位的单克隆和多克隆抗p17抗体。
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