BONE FORMATION COMPOSITION COMPOSED OF MIXTURE OF OSTEOBLAST AND BIO-MATRIX AND ITS MANUFACTURING METHOD

摘要

Provided is a composition for bone formation using a mixture of an osteoblast and a bio-matrix, and a method for preparing the same. The methods comprises isolating osteoblasts from a bone tissue and culturing/proliferating the isolated osteoblasts in DMEM (Dulbecco's Modified Eagle's Medium) or α-MEM (Minimum Essential Medium, Alpha Modification) to prepare an osteoblast suspension; and mixing the resulting osteoblast suspension with a bio-matrix to prepare an osteoblast therapeutic agent. In accordance with the present invention, it is possible to realize a bone formation method, resulting in no clinical graft rejection via injection of an osteoblast and bio-matrix mixture for bone formation into a site where bone formation is sought, and being capable of achieving effective and rapid bone formation via injection of a composition which was shaped to a certain extent, so as to alleviate problems associated with probability of bone tissue formation in unwanted regions resulting from escape of injected osteoblasts from the targeted site for bone formation and then propagation thereof to other sites via the blood stream, which are caused by injection of an osteoblast suspension.