首页>
外国专利>
E. COLI WITH REDUCED GENOME LACKING INSERTION SEQUENCES AND SCARS
E. COLI WITH REDUCED GENOME LACKING INSERTION SEQUENCES AND SCARS
展开▼
机译:E.具有减少的基因组缺失插入序列和标度的大肠杆菌
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention provides a bacterium having a genome that is genetically engineered to be at least 2 to about 20% smaller than the genome of its native parent strain. A bacterium with a smaller genome can produce a commercial product more efficiently. The present invention also provides methods for deleting genes and other DNA sequences from a bacterial genome. The methods provide precise deletions and seldom introduces mutations to the genomic DNA sequences around the deletion sites. Thus, the methods can be used to generate a series of deletions in a bacterium without increasing the possibility of undesired homologous recombination within the genome. In addition, some of the methods provided by the present invention can also be used for replacing a region of a bacterial genome with a desired DNA sequence.
展开▼