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Acetato-N-2-(2-hydroxyethylamino)-ethyl-salicylideneimino(1-)copper and process for cultivation of cyanobacterium Spirulina platensis

机译:乙酰-N- [2-(2-羟乙基氨基)-乙基]-水杨基亚氨基(1-)铜及蓝藻螺旋藻的培养方法

摘要

The invention relates to a new coordinative compound from the class of salicylideneaminoalcoholates of transition metals and to a process for cultivation of cyanobacterium Spirulina platensis with its use as a compound increasing the copper content in the cyanobacterium biomass.It is claimed a coordinative compound acetato-N-[2-(2-hydroxyethylamino)-ethyl]-salicylideneimino(1-)copper of formula:It is also claimed a process for cultivation of cyanobacterium Spirulina platensis, including the cultivation of Spirulina in a nutrient medium containing, g/l: NaNO3 - 2.5; NaHCO3 - 16.8; NaCl - 1.0; K2SO4 - 1.0; K2HPO4 - 0.5; MgSO4·7H2O - 0.2; CaCl2 - 0.04; FeSO4·7H2O - 0.01; EDTA - 0.08; H3BO3 - 0.00286; MnCl2·4H2O - 0.00181; ZnSO4·7H2O - 0.00022; CuSO4·5H2O - 0.00008; MoO3 - 0.000015 and distilled water up to 1 L, at the temperature of 30...32°C, pH 9.5...10.0 and light of 2000...3000 lx. At the same time, on the 2nd day of cultivation into the nutrient medium is added acetato-N-[2-(2-hydroxyetthylamino)-ethyl]-salicylideneimino(1-)copper, in a concentration of 2...4 mg/L, in which the quantity of Cu2+ is 0.47...0.94 mg/L.
机译:本发明涉及一种新的过渡金属的水杨基亚氨基醇盐类配位化合物,并涉及一种养殖螺旋藻的方法,其用作增加蓝藻生物量中铜含量的化合物。要求保护的配位化合物乙酰-N -下式的[[2-(2-羟乙基氨基)-乙基]-水杨基亚胺基(1-)铜:还要求保护蓝藻螺旋藻的培养方法,包括在含有g / l的营养培养基中培养螺旋藻:硝酸钠-2.5;碳酸氢钠-16.8;氯化钠-1.0;硫酸钾-1.0; K2HPO4-0.5; MgSO4·7H2O-0.2;氯化钙-0.04; FeSO4·7H2O-0.01; EDTA-0.08; H3BO3-0.00286; MnCl2·4H2O-0.00181; ZnSO4·7H2O-0.00022; CuSO4·5H2O-0.00008; MoO3-0.000015和最高1 L的蒸馏水,温度30 ... 32°C,pH 9.5 ... 10.0,光2000 ... 3000 lx。同时,在培养的第二天,以2 ... 4 mg的浓度添加乙酸-N- [2-(2-羟乙基氨基)-乙基]-水杨基亚氨基(1-)铜。 / L,其中Cu2 +的含量为0.47 ... 0.94 mg / L。

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