METHOD WITH A WIDE RANGE OF APPLICATIONS, FOR IDENTIFYING MODULATORS OF G-PROTEIN-COUPLED RECEPTORS

摘要

Identifying (M1) compounds (A) that modify, in an organism, the activity of at least one signal transduction pathway that depends on a G protein coupled receptor (GPCR). Cells that contain at least one GPCR-dependent pathway and at least one G protein are treated with test compounds and the qualitative and quantitative effect of the compound on the pathway is determined from a measurement signal that depends on the pathway. Independent claims are also included for the following: (1) (A) identified by (M1); (2) polynucleotides (I) that encode a polypeptide with G protein properties; (3) host cells containing (I) ; (4) method for producing cells containing (I); and (5) proteins (II) having sequences, reproduced, of 359 amino acids (aa) (S2), 353 aa (S4), 353 aa (S6) and 374 aa (S10).