METHOD FOR THE EXTRACTION OF DNA FROM TISSUES OF ANIMAL ORIGIN FOR GENETIC ANALYSIS BY POLYMERASE CHAIN REACTION

摘要

A method for the extraction of DNA from tissues of animal origin for genetic analysis by polymerase chain reaction comprises fermentative method for tissues disintegration. The solution of tripsin is used for proteolytic treatment, the cells are filtered from crude mechanical admixtures, lysed with guanidine thiocyanate, sorption of DNA is carried out on silicon dioxide, and silicon dioxide is double washed off with 70% ethanol.