method for production of antigenic esherihiosis erythrocytic diagnosticum

摘要

A method for production of antigenic esherihiosis erythrocytic diagnosticum consists in extraction of Escherichia antigens, their inactivation, erythrocytes sensitizing and preparation of the diagnosticum. A suspension is prepared enteroinvasive high toxigenic strain of E. coli 0151 "." The disintegration of the cells is performed, the antigen is extracted as a polypeptide complex with hypertonic sodium chloride solution, precipitated with ammonium sulfate. The sheep erythrocytes are sensitized with formalin and loaded with antigen.