Set of primers, probes, method and kit for genetic polymorphism genotipadodel -250g / LIPC to the gene.

摘要

Set of primers, probes, method and kit for genotyping of -250G / A genetic polymorphism of gene LIPC.; The present invention relates to a set of primers, probes, method and kit for genotyping of genetic polymorphisms, and more specifically for genotyping -250G / A genetic polymorphism of LIPC gene by PCR amplification of a DNA sample using the primer set claimed, and the fluorescence detection using the probe assembly claimed. The main advantages of the present invention are rapidly that enables large-scale genotyping as the PCR reaction and detection of the fluorescent signal are simultaneous, allocation automated obtainable genotypes immediately after the reaction, high sensitivity allowing samples genotyped using very low concentrations of DNA, and less risk of contamination being a homogeneous assay.