Dna that codifies the allergen of yellow mustard (sinapis alba) without a3, and its applications. (Machine-translation by Google Translate, not legally binding)

摘要

Dna encoding the yellow mustard allergen (sinapis alba) sin a 3, and its applications. The invention includes the dna encoding the allergen sin a 3 of yellow mustard seeds (sinapis alba), recognized by more than 30% of patients allergic to this biological source. It also includes said recombinant dna cloned and expressed from eukaryotic host cells obtaining the recombinant protein correctly folded and maintaining immunological properties equivalent to those of the natural allergen. On the other hand, the invention relates to a method of isolation and purification of the natural allergen and to a method of purification of the recombinant allergen. Both the purified sin a 3 protein and the recombinant protein, the homologous proteins, the modified forms derived therefrom, the derivative fragments containing at least one allergenic epitope, can be used in the elaboration of allergy diagnosis methods as well as in the development of immunotherapy systems. (Machine-translation by Google Translate, not legally binding)