Method for retroviral packaging cell lines, producing a retroviral supernatant of high transduction efficiency.

摘要

This invention describes a method for obtaining a cell encapsulation retroviral recombinant retroviral vectors CAPABLE OF PRODUCING, AND RECOMBINANT CELL Encapsulation obtained by the process. Also disclosed a method for producing retroviral particles recombinants, obtained by introducing into cells Encapsulation obtained according to the methods described herein, a retroviral vector recombinant SPREADING producing cells RESULTING under favorable conditions for production and secretion of supernatant retroviral vector . Retroviral supernatants produced by these methods are also claimed herein. This invention further describes a method for selecting a retroviral vector supernatant for obtaining high transduction efficiency, and methods for producing retroviral vector supernatant for transducing cells with high EFICACIAEN APPLICATIONS OF GENE THERAPY.