New chromogenic substrates for the detection of hydrolases.

摘要

Process for detecting a peptidase enzymatic activity, characterized in that it consists in: - putting at least one substrate consisting of at least two molecules, a first molecule consisting of a marker part nonchromogenic associated with at least a portion specific target of the enzyme, being formed, said marker section nonchromogenic, by aminobenzene or a derivative thereof, ** ** Formula and a second molecule consisting of a part marker nonchromogenic consisting of α-naphthol or a its derivatives, ** ** Formula in the presence of at least one type of microorganisms such as bacteria contained in a test sample to be testing, without the addition of oxidase - expect bacteria hydrolyze the (the) substrate (s), and - detecting the formation of a colored complex from the oxidative coupling of two labeling portions, corresponding, said colored complex, the molecule ** Formu the ** Formulas thereof during which: · the radial R1, is consisting of OH, SH, or N (X) (Z) wherein X and / or Z, are selected from H, CH3, CH2CH3, N (CH3) 2; N (CH2CH2OH) 2, and ** ** Formula · the radical R10 is chosen from H, Br, Cl, I, and groups of atoms such as SH, that may be removed during the oxidative coupling each radical R2 · and to R9, R11 and R12 is selected from H, OH, Br, Cl, and I, CH3, CH2CH3, OCH3, OCH2CH3 and COOH.