Detection of tumor stem cells and tumor cells in epithelial-mesenchymal transition in body fluids of cancer patients.

摘要

Solid phase method for isolation, enrichment and / or detection for isolating, enriching and / or detecting tumor stem and / or tumor cells in epithelial-mesenchymal transition as predetermined cells from a sample of body fluid containing such predetermined cells for at least one of the group comprising staging, prognosis and therapeutic guidance. said method comprising a selection step for selection or enrichment of said predetermined from the sample cell in the sample is contacted with a solid surface for preferential binding of said predetermined to the solid surface cells and subsequently the sample removed from the solid surface in a washing step, wherein in said selecting step for selection or preferential enrichment in tumor cells, the sample contains a polyol for at least one of contacting the sample with the solid surface and washing step and in a detection step detecting in said cells, preferably are selected or enriched by said selecting step, the presence or absence of expression of at least one marker associated with at least one group comprising hemocytoblasts tumor and tumor cells in epithelial-mesenchymal transition.