PURIFICATION OF TACI-FC FUSION PROTEINS USING THE FAT BODY TECHNOLOGY.

摘要

A method for purifying a TACI-Fc protein, comprising the steps of: a) mixing a solution comprising fatty bodies associated with a protein A with a sample comprising said TACI-Fc protein in order to obtain a final mg ratio of TACI-Fc protein per mg of dry weight of fatty bodies, within a range from 1 to 5.5; and b) separating said fatty bodies with protein A from said TACI-Fc protein by adding an aqueous solution having a pH within a range of 3.5 to 3.9, wherein the solution comprising TACI-Fc obtained at the end of step (b), comprises more than 93%, 94%, 95% or 96% of dimers of TACI-Fc.