MUTEINS OF HUMAN NEUTROPHIL GELATINASE-ASSOCIATED LIPOCALIN AND RELATED PROTEINS

摘要

Disclosed is a method for generating a mutein of a protein selected from thegroup consisting of human neutrophil gelatinase-associated lipocalin (hNGAL),rat .alpha.2- microglobulin-related protein (A2m) and mouse 24p3/uterocalin(24p3), said mutein having detectable affinity to a given target. The methodcomprises the step of subjecting the protein to mutagenesis at one or more ofthe sequence positions which correspond to the sequence positions 33 to 54, 66to 83, 94 to 106, and 123 to 136 of hNGAL, resulting in one or more mutein(s)of the protein. Also disclosed are muteins obtainable by this method.