PRIMER EXTENSION BASED METHOD EMPLOYING NUCLEOTIDES LABELLED VIA CLEAVABLE LINKERS

摘要

The present invention relates to a method for determining the sequence of anucleic acid molecule comprising the steps of: a) providing a single-strandedform of said nucleic acid molecule; b) hybridizing a primer to said singlestranded form of said nucleic acid molecule to form a template/primer complex;c) enzymatically extending the primer by the addition of a polymerase and amixture of at least one nucleotide and at least one labeled derivative of theat least one nucleotide, wherein the at least one labeled derivative of the atleast one nucleotide comprises a label linked to the nucleotide via acleavable link and wherein the amount of labeled derivative of the at leastone nucleotide in said mixture of the at least one nucleotide and the labeledderivative of the at least one nucleotide is within the range of 1-50 mole-%,1-40 mole-%, 1-30 mole-%, or 1-20 mole-%. d) determining the type ofnucleotide added to the primer; and e) repeating steps c) to d) at least once.