MULTI-FUNCTIONAL NANOMATERIAL BASED ON MODIFIED ARCHAEAL HALOBACTERIUM SALINARUM FLAGELLA (EMBODIMENTS) AND A METHOD FOR PRODUCING SAME

摘要

The invention relates to the field of biotechnology and genetic and protein engineering, more specifically to nanotechnological uses of modified archaeal H. salinarum flagella for forming active surfaces on flexible and rigid substrates or capsules in the fields of electronics, diagnostics, medicine and environmental cleansing. The subject matter of the invention is a method for producing a nanostructured material based on modified archaeal H. salinarum flagella, which includes transforming archaeal cells with a recombinant plasmid, cultivating said cells, isolating the flagella and modifying the surface thereof with metal ions or nanoparticles. The plasmid architecture contains recombinant genes for the synthesis of the A1 and A2 flagellins that form a flagellum. Furthermore, the sequence of the flagellin A1 or A2 or the sequences of flagellins A1 and A2 contain at least one peptide insert for the selective binding of metal ions or nanoparticles. The peptide insert is made in the flagellin A1 in the region between the first and second glycosylation sites, i.e. between position 86 and position 96 SEQ ID NO: 2; and the peptide insert is made in the flagellin A2 in the region between the first and second glycosylation sites, i.e. between position 82 and position 92 SEQ ID NO: 3. The length of the peptide insert is selected from between 5 and 60 amino acids. A further subject matter of the invention is a multi-functional nanomaterial based on modified archaeal H. salinarum flagella. In the first embodiment, the nanomaterial is in the form of a fibre structure containing over 50% archaeal flagella with a length of more than 1000 nm. In the second embodiment, the nanomaterial is in the form of a cluster structure containing over 50% flagella with a length of less than 500 nm.