METHODS FOR DETERMINING CONFORMATIONAL CHANGES AND SELF-ASSEMBLY OF PROTEINS

摘要

The invention relates to methods for measuring conformational changes and self-assembly/aggregation of proteins, especially the formation of amyloid fibrils, using conjugated polyelectrolytes. The conjugate polyelectrolyte is exposed to the protein whereby the conjugated polyelectrolyte and the protein of interest interact, and a change of a property of said polyelectrolyte in response to conformational changes of the protein is observed. The detected change is used to determine different conformations of said protein, especially the formation of amyloid fibrils.