RECOMBINANT EXPRESSION VECTOR FOR EXPRESSING BRAZZEIN, AND NOVEL MULTIPLE MUTATION BRAZZEIN VARIANT HAVING GREATER SWEETNESS

摘要

The present invention relates to a recombinant expression vector for expressing brazzein and to a method for the mass production of brazzein using the vector, in which a brazzein expression system is constructed in Kluyveromyces lactis, which is a lactose-fermenting yeast, and particularly, in which an optimum condition is established to achieve significantly superior expression than conventional E. coli or yeast expression systems, thus enabling the mass production of brazzein. In addition, the present invention relates to a novel biquadratic brazzein variant having greater sweetness, which has the same thermal stability as that of conventional brazzein, and a sweetness that is at least 15 to 50 times higher than that of conventional brazzein. Therefore, the brazzein variant of the present invention can be used together with other sweeteners such as sugar (sucrose), and further less brazzein variant may be used as a substitute for the same amount of sugar, and can be variously used as additives in food production.