Screening method of apoptosis controlling agent using yeat lacking GDH3

摘要

The present invention using the gdh3 deficient yeast and to a method of screening for regulators of apoptosis. More specifically, the present invention is gdh3 the transgenic yeast deficient in protein or nucleic acid encoding a candidate gdh3 Defect treatment with the test substance in yeast is cultured and then the candidate protein or the test substance by measuring the apoptosis activity of the yeast under thermal stress according to the screening method of the apoptosis regulators, characterized in that to determine whether the apoptotic activity is controlled. ; of the present invention gdh3 deficient yeast because it has a high sensitivity to apoptosis induced by heat and apoptosis regulators using the effective strain can be screened.