A MULTIFLEX REAL TIME PCR FOR DETECTING FOOD POISONING PHATHOGEN BACTERIUM

摘要

PURPOSE: the food poisoning bacteria in real-time PCR is provided to confirmation Staphyllococcus staphylococcus aureuses, vibrio parahaemolytious, salmonella typhimurium. ;CONSTITUTION: Multiplex PCR detects staphylococcus aureus, and vibrio parahaemolytious, salmonella typhimurium includes: to prepare template DNA from staphylococcus aureus, vibrio parahaemolytious, salmonella typhimurium in the first step; The step of selecting primer; It executes the pcr template DNA of multiple steps and uses the primer set; With detection staphylococcus aureus, vibrio parahaemolytious, from salmonella typhimurium PCR product. ;The 2011 of copyright KIPO submissions