PURPOSE: A method for inducing differentiation of human embryonic stem cells to cardiac myocytes is provided. CONSTITUTION: A method for inducing differentiation of human embryonic stem cells to cardiac myocytes comprises: a step of culturing the embryonic stem cells in a medium containing activin A for one day after differentiation; and a step of culturing the embryonic stem cells in a serum-free medium containing BMP2 for 1-5 days. The culture is performed by direct differentiation without formation of embryo bud. The concentrate of activin A is 50-100 ng/ml.
展开▼