METHOD FOR DETECTING MICROCYSTIN USING PAPER CHROMATOGRAPHY AND CHEMICAL LUMINESCENCE

摘要

PURPOSE: A method for detecting microcystin using paper chromatography and chemical luminescence is provided to simply and conveniently detect microcystin.;CONSTITUTION: A method for detecting microcystin comprises: a step of injecting a liquid sample containing analytes with microcystin on a strip comprising a sample pad and chromatography medium; a step of drying the strip and fixing the analyte in the medium; a step of injecting an enzyme conjugate which causes luminescent reaction with a monoclonal antibody to induce antigen-antibody reaction with the microcystin; and a step of adding a light emitting solution for luminescent reaction. The medium is a nitrocellulose.;COPYRIGHT KIPO 2012