Mutated Nucleotide Sequences of Batroxobin, Mutated ??Factor Secretion Signal Sequence and Processes for Preparing Batroxobin Using the Same

摘要

The invention bat lock sobin (batroxobin) having a specific sequence relates to the vectors and transformants comprising the coding nucleotide sequence and / or modified α- factor secretion signal sequence. Bet of the present invention sobin rock-coding nucleotide sequences of yeast, in particular Pichia expression is very good in pass efficiency Laboratories, bats Rock sobin of natural (natural-occurring) - compared to the coding sequence 4-13 times increased yield It allows to obtain a recombinant Bet sobin rock. When expressed as a fusion protein coding type using the nucleotide sequence and the modified α- factor secretion signal sequence at the same time, the bat of natural rock sobin-present invention of recombinant Bet rock sobin at an increased yield up to 20 times compared to the coding sequence and to obtain a lock sobin bat. In addition, recombinant Bet rock sobin prepared using the sequences of the present invention is excellent in activity as compared with the batt of natural rock sobin, is excellent in stability. ; Rock sobin bat, genes, yeast, Pichia protein expression, production, yield