Biomarker for identification of exposure to Polycyclic Aromatic Hydrocarbons and the method of identification using thereof

摘要

PURPOSE: A biomarker for detecting exposure to polycyclic aromatic hydrocarbons(PAHs) is provided to usefully monitor and determine contamination of PAHs and figure toxicity mechanism caused by the PAHs. CONSTITUTION: A biomarker for detecting exposure to polycyclic aromatic hydrocarbon(PAHs) contains a gene of which expression is decreased or increased by the exposure of the PAHs. A DNA microarray chip for detecting the exposure to the PAHs comprises oligonucleotide including entire or partial biomarker gene sequence and its complementary oligonucleotide. A method for detecting the exposure of the PAHs using the biomarker comprises: a step of isolating RNA from sample-derived human somatic cells as an experimental group and normal somatic cells as a control group; a step of synthesizing RNA to cDNA and labeling the experimental group and control group with different colors; a step of hybridizing the cDNA on the DNA microarray chip; a step of analyzing the DNA microarray chip; and a step of comparing the expression level between the experimental group and control group.