Human infectious diseases-related pathogen differential diagnosis and simultaneous antibiotics resistance analysis, multiplex kit and chip comprising same

摘要

A method for diagnosis of infectious disease-related microorganisms is provided to detect the infection of infectious disease-related microorganisms and analyze their genotype and antibiotic resistance simultaneously through one-stop analysis, so that abuse of antibiotics is reduced and treatment period and expense are saved. A method for diagnosis of infectious disease-related microorganisms comprises the steps of: amplifying each disease group of the infectious disease-related microorganisms through multiplex-PCR(polymerase chain reaction) by using oligonucleotide primers of SEQ ID NOs:1 to 90; inducing hybridization of the PCR products with a probe corresponding to the disease, immobilized on a chip; and determining the hybridization, wherein the disease groups include urinary tract infection, rhinosinusitis, sexually transmitted disease, human papilloma virus, periodontitis and Mycobacteria infection, wherein the probe has an oligonucleotide selected from SEQ ID NOs:91-216.